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20S 免疫蛋白酶体 
(无标签，人类)
通过无标签分级法从人类脾脏中纯化

50微克-咨询价格

Immuno20S figure.png
SDS-PAGE i20S Nov2024_1.png

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20S immunoproteasome purified from human spleen. Human 20S immunoproteasomes were separated from standard 20S to apparent homogeneity. (A) Chromatogram depicting the separation. (B) Immunoblotting of 20S immunoproteasome (LMPT-7) and standard 20S (MB1). (C) Coomassie stained 12% SDS-PAGE following a final column purification step. A clear separation between immunoproteasome 20S and standard 20S purified from human spleen is achieved. 

产品详情

来源：通过无标签分级法从人体脾脏中纯化。表现出最高酶活性，可溶且均匀的。
配方：于20mM Tris-HC，pH 7.2的缓冲液中，包含1mM 叠氮化钠，1mM DTT和50%甘油。

缓冲液成分可根据客户需求进行调整。
运输方式：干冰运输
长期储存： -80°C

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科学背景

真核蛋白酶体包含7个α型和7个β型亚基。在干扰素-γ（IFN-γ）刺激下，三个活性β亚基：β1（Y）、β2（Z）和β5（X），会被其对应的免疫亚基部分：β1i（LMP-2）、β2i（MECL-1）和β5i（LMP-7）所替换。β型亚基的替换会使得蛋白酶体的底物特异性发生改变。已有部分研究表明，20S蛋白酶体的类胰凝乳蛋白酶和类胰蛋白酶样活性增加。同时也有其他报告指出，在此类处理后20S蛋白酶体活性没有变化，甚至呈现出下降的情况。

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我们提供的20S免疫蛋白酶体为高度纯化产品。所有起始材料均已接受专业检测，结果显示其对乙型肝炎表面抗原、人类免疫缺陷病毒1型抗原、人类免疫缺陷病毒1型和2型抗体及丙型肝炎病毒均为阴性。

 

使用与注意事项

避免反复解冻

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产品参考文献
1) Conformational maps of human 20S proteasomes reveal PA28- and immuno-dependent inter-ring crosstalks, Jean Lesne and Julien Marcoux et al.; Nat Commun.,11: 6140, (2020), Full Text

 

2) Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders, Scott Ugras and Harry Ischiropoulos et al.; EBioMedicine., 31: 307–319, (2018), Full Text

 

3) The presence of prolines in the flanking region of an immunodominant HIV‐2 gag epitope influences the quality and quantity of the epitope generated, Sabelle Jallow and Sarah L. Rowland‐Jones et al.; Eur J Immunol., 45(8): 2232–2242, (2015), Full Text

 

4) Differential Roles of Proteasome and Immunoproteasome Regulators Pa28αβ, Pa28γ and Pa200 in the Degradation of Oxidized Proteins, Andrew. M. Pickering and Kelvin. J. A. Davies; Arch Biochem Biophys., 523(2): 181–190, (2012), Full Text

 

5) Pivotal Advance: Protein synthesis modulates responsiveness of differentiating and malignant plasma cells to proteasome inhibitors, Simone Cenci and Paolo Cascio et al. J Leukoc Biol., 92(5):921-31, (2012), Full Text

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